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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 13;391(1):299–304. doi: 10.1016/j.bbrc.2009.11.053

Figure 4. Promotion of muscle differentiation by overexpressed NS is dependent on p53.

Figure 4

(A) Immunostaining of p53−/− myoblasts infected with the empty or the NS-expressing retrovirus. Anti-6xHis tag antibody was used to detect NS. Bar, 10 μm.

(B) Cell proliferation profiles of p53−/− myoblasts infected with the empty or the NS-expressing retrovirus.

(C) Immunostaining of p53−/− myoblasts during differentiation after infection with the empty or the NS-expressing retrovirus. Bar, 40 μm.

(D) Mean + SEM of the ratios between the number of DAPI-positive nuclei in MHC-positive cells and the total number of DAPI-positive nuclei shown in (C). One hundred nuclei were counted in each experiment.