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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Biochem Pharmacol. 2009 Oct 14;79(5):706–714. doi: 10.1016/j.bcp.2009.10.006

Figure 5. Proteasomal regulation of promoter-associated p65/RelA limits inflammatory gene transcription induced by PV.

Figure 5

(A). ILU-18 cells were either untreated or treated with PV (100μM) for 2 and 4 hours, with or without pretreatment with Acla (0.25μM) for 2 hours. ChIP assay employing anti-p65 was performed using immunoprecipitated DNA amplified with primers specific for the promoter region of the genes, MCP-1, IκBα, and COX-2. ChIP assay employing α-HA (irrelevant antibody) in cells treated with Acla+PV served as a specificity control.

(B). Total RNA was extracted from ILU-18 cells treated with TNFα (20ng/mL) or Acla (0.25μM) for 4 hours or PV (100μM) for 4 hours with or without pretreatment with Acla for 2 hours. Equal amounts of RNA were analyzed by real-time PCR. Fold induction represents treatment-induced COX-2 expression relative to basal COX-2 expression in untreated cells; representative data from two independent experiments are presented. Statistically significant differences are denoted by **.