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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 29;391(1):895–898. doi: 10.1016/j.bbrc.2009.11.159

Figure 2. Changes in the protein contents of FTase and GGTase subunits in INS 832/13 cells and isolated rat pancreatic islets exposed to basal or stimulatory glucose.

Figure 2

Panel A: INS-832/13 cells and isolated rat pancreatic islets were cultured overnight in low glucose-low serum media. Cells were further incubated in presence of either low glucose [2.5 mM; LG] or high glucose [20 mM; HG] for 45 min. Homogenate proteins were separated by SDS-PAGE, transferred to a membrane and probed with corresponding antibodies for detection of FTase/GGTase 1- α, FTase β and GGTase 1-β subunits. A representative blot from two studies is shown here. Panel B: Relative intensities of protein bands from experiments described under Panel A are quanitiated by densitometry and plotted. Pooled data from two separate studies are given.