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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Dev Biol. 2009 Nov 6;337(2):303–312. doi: 10.1016/j.ydbio.2009.10.046

Figure 3. Loss of 14-3-3 results in nuclear accumulation of Yki.

Figure 3

(A and B) Low (A) and high (B) magnification view of a wild type wing disc immunostained with an anti-Yki (green) antibody. Yki is primarily localized in the cytoplasm.

(C-D″) Low (C) and high (D-D″) magnification view of a wing disc expressing both UAS-14-3-3ε RNAi and UAS-14-3-3ζ RNAi together with UAS-P35 using MS1096 and immunostained with anti-Yki antibody (green in C, D and D″) and 7-AAD (red in D′ and D″) to label the nuclei. The wing disc is oriented with anterior to the left and ventral up. Severe knockdown of both 14-3-3 isoforms in the dorsal region of the wing pouch resulted in nuclear accumulation of endogenous Yki.