Figure 4. Multiple phosphorylation events contribute to Yki regulation.
(A-D′) Side views (A-D) or dorsal views (A′-D′) of adult eyes expressing UAS-Myc-Yki (A and A′), UAS-Myc-YkiS168A (B and B′), UAS-Myc-YkiS111,250A (C and C′) or UAS-Myc-Yki3SA (D and D′) with GMR-Gal4.
(E) S2 cells were transfected with the indicated Yki constructs together with Sd and an Sd-luciferase reporter gene, with or without Hpo and Wts expressing constructs. Cell lysates were subjected to dual luciferase assay. Error bars indicate standard deviation (triplicate wells). Numbers indicate degrees of suppression of Yki/Sd activity by Hpo/Wts.
