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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Dev Biol. 2009 Nov 6;337(2):303–312. doi: 10.1016/j.ydbio.2009.10.046

Figure 7. Restriction of Yki nuclear localization by Hpo signaling depends on nuclear export.

Figure 7

S2 cells were transfected with Myc-tagged wild type or mutant forms of Yki together with HA-Sd and with or without a Flag-Hpo expression construct. Cells were treated without or with LMB at a concentration of 20 nM for 4 hours, followed by immunostaining with Myc and HA antibodies. For each Yki construct, 100 cells were randomly selected and Yki subcellular localization was determined using confocal microscopy. C>N: cells contain higher levels of Yki in the cytoplasm than in the nucleus. C=N: cells contain Yki equally distributed in cytoplasm and nucleus. N>C: cells contain higher levels of Yki in the nucleus than in the cytoplasm.