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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Nov 24;1797(2):285–295. doi: 10.1016/j.bbabio.2009.11.005

Fig. 1. Protein glutathiolation by diamide.

Fig. 1

Analysis of protein-glutathione (PSSG) adduct formation by Western blotting: (A) Representative Western blot of PSSG adducts: Cells were treated with diamide (0–1 mM) for 1 h. Cell lysate proteins were then separated by SDS-PAGE followed by chemifluorescent Western blotting using anti-protein-glutathione (anti-PSSG) antibodies. (B) Group data from panel A. n = 3 per group; *p<0.05 vs. cells not treated with diamide.