Fig. 6. Bioenergetic function in glutathione-depleted cells treated with diamide.
Extracellular flux analysis plots of oxygen consumption and extracellular acidification: (A) Cells were treated without or with 100 µM BSO for 24 h (open squares), and baseline oxygen consumption rates (OCR) were recorded. Diamide (dotted lines) was then injected to 0.1 mM final concentrations where indicated, and the OCR were followed for approximately 300 min. (B) Glycolytic flux in glutathione-depleted cells treated with diamide: Cells were treated as described in panel A and rates of extracellular acidification were measured. N = 5 per group.
