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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 10;391(1):176–181. doi: 10.1016/j.bbrc.2009.11.027

Figure 2. HSV-1 binds and surfs along filopodia.

Figure 2

(A) Time course of K26GFP virus (green) surfing (arrow) on a single Vero cell at indicated time points. Cells were infected at an approximate MOI of 200. (B) Representative binding of K26GFP virus to filopodia. To quantify virus binding, the average fluorescent intensity of a selected region of interest on filopodia was plotted over time. (C) Tracking of viral particles demonstrates an average particle speed of 1.5 μm/min. To quantify viral surfing speed, 8 to 10 particles per cell were tracked every 30 s and the distance traveled was plotted over time.