Skip to main content
. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 10;391(1):176–181. doi: 10.1016/j.bbrc.2009.11.027

Figure 3. Heparan sulfate and gB are important for attachment to filopodia and surfing.

Figure 3

(A) Anti-Heparan sulfate staining of Vero and CHO-K1 cells. As a negative control, primary antibody was omitted. FITC conjugated secondary anti-mouse IgG (SIGMA) was used in 1:200 dilution. (B) Western Blot Analysis confirming gB and gD presence in cell lysates. (C) Quantification of the average green intensity around the cell membrane and filopodia for gB and gD. 20 μg of total protein from gB and gD lysates were taken and incubated with monoclonal antibodies (Virusys Corp and Abcam, respectively) before using as probes on cells. The figure represents 3 independent experiments (n=11). Data are presented as means with error bars showing standard deviations. (D) Quantification of Heparinase treated cells and gB null virus. Average green intensity at filopodia was measured after 1 h of treatment with the enzyme or incubation with the mutant virions. Error bars show standard deviations. (E) Quantum dots (red) conjugated to gB via an antibody were used for demonstration of binding of gB to filopodia and its ability to surf on HeLa cells.