Figure 3. Tsc2^+/− mice have defects in topographic mapping of contralateral projections in dLGN.

(a) Representative series of fluorescent micrographs showing termination territories in contralateral dLGN from DiI-labeled ventro-temporal RGCs of Tsc2^+/+ versus Tsc2^+/− littermates at P19. Anterior is to the left and posterior to the right in each series of sections. Dashed lines represent the borders of dLGN. Arrows indicate DiI labeled RGC termination zones. Insets represent tracings of the flat-mounted retina and injection site for each mouse. OT: optic tract; D: dorsal; V: ventral; T: temporal; N: nasal; and L: lateral. (b) Schematic drawings and colored dots show the position and area of DiI labeled termination in each corresponding dLGN section from wild-type (in red) and Tsc2^+/− (in green) littermates. In Tsc2^+/−, the lower border of the termination extended erroneously into the area that is innervated by the ventral-nasal RGCs in wild-type mice⁸. Scale bar represents 100 µm. (c) Quantification of the percentages of DM to VL extension to length of dLGN along the same axis. Data are represented as mean ± SEM (19.1 ± 2.1% in wild-type, n = 4 mice; and 50.3% ± 9.7 % in Tsc2^+/− littermates, n = 6 mice. **: P < 0.01 by t-test).