Skip to main content
. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Exp Neurol. 2009 Oct 13;221(1):86–97. doi: 10.1016/j.expneurol.2009.10.005

Figure 1. Expression of WT- or A53Tα–syn in conditional Tg mice.

Figure 1

A, To generate conditional Tg mice (tTA/WTα–syn, or tTA/A53Tα–syn), Tg activator line (CaMKIIα–tTA) was crossed with responder line (tetP-WTα–syn, or tetP-A53Tα–syn). Doxycycline (dox) prevents tTA from binding to tetP thus repressing Tg expression (tet-off). B, Immunoblot analyses of α–syn expression in four different Tg lines (lines 3 and 7 for WTα–syn, lines 9 and 33 for A535α–syn) (n=3). 20 µg of total forebrain lysates prepared from cortical and subcortical tissues (6 week-old), were used to detect α–syn with LB509 (specific for human α–syn) and SNL-1 (specific for human and mouse α–syn) antibodies. α-tubulin (α–Tub) was used for an internal loading control. PrP-α–syn mice (line M83) (Giasson et al., 2002) were used as positive controls. C, Immunoblot analyses of α–syn expression in different brain regions. Six different brain regions were dissected out from nTg, tTA/WTα–syn and tTA/A53Tα–syn mice (postnatal day 21, P21), and their lysates (20 µg per lane) were compared for α–syn expression using LB509 and SNL-1 antibodies. OB, olfactory bulb; CTX, cerebral cortex; HP, hippocampus; SubCtx, subcortical regions (basal ganglia, thalamus, hypothalamus etc); CBL, cerebellum; BS, brain stem.