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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Exp Neurol. 2009 Oct 13;221(1):86–97. doi: 10.1016/j.expneurol.2009.10.005

Figure 3. α–syn overexpression leads to massive reduction in number of neuronal cells in the hippocampal dentate gyrus (DG).

Figure 3

A–H, Hematoxylin and eosin (H & E) staining of coronal sections of the hippocampal DG at P14 (A,B,E,F) and P21 (C,D,G,H). At P14, tTA/A53Tα–syn (F) mice show mild atrophy in the DG compared to nTg littermates (E). At P21, severe atrophy in the DG was observed in both tTA/WTα–syn (D) and tTA/A53Tα–syn (H) mice compared to nTg littermates (C,G). Each inset is a magnified image of black boxed area. I, Immunoblot showing α–syn expression in tTA/A535α–syn mice at P21 (total brain lysates) with or without doxycycline (dox) treatment (E0.5~P21) compared to littermate control. J–L, Prox1 immunostaining of the DG (P21) in A53Tα–syn (J), tTA/A53Tα–syn (K), and tTA/A53Tα–syn-dox (dox treated from E0.5~P21) (L) mice. M–T, Double immunofluorescent staining of nTg (M–P) and tTA/A535α–syn (Q–T) DG sections (P14) with nestin (N,R) and neuN (O,S) antibodies. Insets in merged images (P,T) are magnified images of boxed areas. DAPI staining was used for nuclear counter staining (M,Q). U, Quantification of NeuN staining (P14) in the DG as shown in O, S (n=2~3, **p < 0.01 two tail t test). Scale bars in A, J, M = 100 µm.