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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Exp Neurol. 2009 Oct 13;221(1):86–97. doi: 10.1016/j.expneurol.2009.10.005

Figure 4. Increased DG neuron death, not decreased proliferation, is responsible for reduced number of neuronal cells in tTA/A53Tα–syn mice.

Figure 4

A–D, Immunostaining of hippocampal sections using an antibody against phosphorylated-histone 3 (p-H3), mitosis marker. Both at E17.5 (A,B) and P14 (C,D), the number of p-H3 positive cells is not decreased in tTA/A535α–syn (B,D) compared to littermate control (A,C). E–F, Immunostaining of DG sections (P14) with antibody against cleaved-caspase 3, apoptotic marker. The number of cleaved-cas 3 positive cells is increased in tTA/A53Tα–syn (F) compared to littermate control (E). Note the distribution of cleaved-cas 3 positive cells in tTA/A53Tα–syn (arrows in the inset in F) is not limited to the subgranular zone where normal apoptotic cells (arrows in the inset in E) are observed in littermate control. At P7, no differences in cleaved-cas 3 staining were detected between tTA/A53Tα–syn and littermate control (data not shown). G, Quantification of cleaved-cas3 positive cells in the same level of the DG sections from bigenic mice and littermate controls (two representative sections from three animals each). H, Immunoblot analyses using hippocampal lysates (P21) probed with p-H3 and cleaved-cas3 antibodies. α–Tub was used for loading control.

Scale bars in A, C = 100 µm; E=20 µm.