Figure 5. Synaptic proteins are reduced in the tTA/A53Tα–syn hippocampus.

A, Representative immunoblot results comparing synaptic protein levels between tTA/A535α–syn mice and littermate controls, at P7, P14 and P21. α–Tub was used for internal loading control. B, Quantification of synaptic protein levels from the immunoblots. Duplicate~quadruplicate immunoblots were used for quantification and band intensity of each protein was corrected relative to that of α–Tub in the same blot. Data are shown as Mean ± SEM (nTg, n=2~4; tTA/A53Ta-syn, n=4~8; *p < 0.05, **p < 0.01, ***p < 0.005 two tail t test)