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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Arch Biochem Biophys. 2009 Oct 30;493(2):184–191. doi: 10.1016/j.abb.2009.10.014

Fig. 1.

Fig. 1

(A) Rapid-scan absorbance data for the reaction of CYP153A6 with mCPBA at 25 °C, pH = 8.0, P450, 7.1 μM; mCPBA, 150 μM. Selected spectra from 0 to 0.3 s are shown. Inset, data recorded at indicated wavelengths. (B) SVD analysis of the rapid scan data recorded for the reaction in (A). Model used was A→B→C→D with k1 = 91 s-1, k2 = 22. 5 s-1, k3 = 1.5 s-1. A (–○–○–); B (– – –); C (–x–x–); D (--△--△-).

Inset, expansion of data in the visible region. (C) Molar fractions of the different forms of CYP153A6 on the reaction pathway from (B).