Fig. 7.
Double mixing experiments showing spectral changes upon mixing CYP153A6 (~28.8 μM before mixing) with mCPBA (600 μM mCPBA before mixing) at pH 7.4, 25 °C in the first mix, and ascorbic acid (20 mM final concentration) in the second mix (age time 55.4 ms). To show spectral changes more clearly, the peak of the Soret band has been expanded. Inset, absorbance traces at selected wavelengths.
