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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Dev Biol. 2009 Nov 10;337(2):335–350. doi: 10.1016/j.ydbio.2009.11.008

Figure 1. Ventralized embryos express residual sox2 and sox3.

Figure 1

X. laevis embryos were injected in both blastomeres at the two cell stage with β-catenin MO, then raised to the stage indicated, fixed in MEMFA, dehydrated and stained by in situ hybridization for expression of sox2 (A) or sox3 (B). Uninjected control sibling embryos are shown at right for each stage. For β-catenin morphants, a ring of sox2 expression is evident around the blastopore between stages 14 and 16, which fades and has disappeared by stage 19. The same pattern is seen for sox3. Embryos are shown in dorsal views with anterior to the top.