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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Dev Biol. 2009 Nov 10;337(2):335–350. doi: 10.1016/j.ydbio.2009.11.008

Figure 4. X. laevis FCN morphants do not express sox2.

Figure 4

X. laevis embryos were injected in each blastomere at the 2 cell stage with 20ng each of follistatin, chordin, and noggin MOS. Embryos were raised to the stage indicated, fixed in MEMFA, and stained for expression of sox2 or epidermal cytokeratin. A) Some embryos expressed low levels of sox2, but this expression was not seen in a ring. Most FCN morphants do not express sox2. The fraction of embryos falling into each class of sox2 expression are shown in the lower right corner. B) Cytokeratin is expressed strongly in FCN morphants at all stages assayed, but is excluded from the blastopore and adjacent tissue (22/24 embryos with strong circumferential expression). C) Xbra expression is expanded in neurula stage FCN morphants. Embryos are shown in dorsal views with anterior to the top.