Figure 5. sox2 has slightly different endogenous expression in X. tropicalis and X. laevis.
X. tropicalis or X. laevis embryos were raised to the stage indicated, fixed in MEMFA and analyzed by in situ hybridization for expression of sox2. In X. tropicalis, sox2 expression closes posteriorly around the blastopore, and is tightly adjacent to the blastopore margin. In X. laevis, posterior circumblastoporal expression of sox2 is weak. All embryos are shown in dorsoposterior views with anterior to the top; the embryos have been rotated slightly to clearly show the blastopore.
