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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Arch Biochem Biophys. 2009 Oct 20;493(2):135–142. doi: 10.1016/j.abb.2009.10.006

Fig. 1.

Fig. 1

Cleavage of pETBlue plasmid DNA by HOCl. Conditions: 120 ng of purified plasmid was incubated at 37 °C with the indicated amounts of HOCl in 10 µL PBS for 10 min, after which excess Na2S2O3 was added to quench any remaining oxidant and the samples were immediately run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows a standard 1 kB DNA ladder.