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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Arch Biochem Biophys. 2009 Oct 20;493(2):135–142. doi: 10.1016/j.abb.2009.10.006

Fig. 2.

Fig. 2

Cleavage of pETBlue plasmid DNA by ONOOH. Conditions: 100–120 ng plasmid in 10 µL phosphate buffer, pH 7.4, mixed at ambient temperature with the indicated amounts of peroxynitrite, then run immediately on 0.8% agarose. Upper gel: 0.1 mM added bathocuproin sulfonate (BCS); lower gel: 0.1 mM added Cu(NO3)2. Lane 9 contains the 1 kB DNA ladder.