Fig. 4.

Reaction of plasmid DNA in pETBlue-transformed NovaBlue cells exposed to ONOOH. Conditions: ~3×109 cells/mL in 50 mM phosphate, pH 7.4, under an argon atmosphere mixed with the indicated amounts of peroxynitrite at ambient temperature, after which the plasmid was isolated and run on 0.8% agarose.