Fig. 7.

5-Chlorouracil isolated from genomic DNA in HOCl-treated E. coli 11775. Conditions: ~8×107 cells/mL incubated 6 h in PBS at 37 °C with the indicated concentrations of HOCl, after which the DNA was isolated and analyzed mass spectrometrically for 5-chlorouracil content as described in Experimental Procedures. The arrow indicates the HOCl concentration corresponding to LD90 (12 µM). Data are averages of 3–6 experiments; error limits are average deviations from the mean values.