Table 1.
Isotopomer analysis of fragments formed by C-C bond cleavage at the main chain backbone of aldose-MOA (D-glucose-MOA) and ketose-MOA (D-fructose-MOA) observed in GC/MS EI mode spectra. (See inserts of Figure 1a and 1b for suggested fragmentation patterns).
| Methyloxime-peracetate | Fragments Observed | Isotopomer m/z values | |||
|---|---|---|---|---|---|
| D-glucose | [1,2-13C2] D-glucose | [U-13C6] D-Glucose | [6-13C1] D-glucose | ||
| Glucose | C1-C6 | m/z = m | m/z = m+2 | m/z = m+6 | m/z = m+1 |
| C1-C5 | 347 (m) | 349 (m+2) | 352 (m+5) | 347 (m) | |
| C1-C2 | 131 (m) | 133 (m+2) | 133 (m+2) | 131 (m) | |
| C3-C6 | 289 (m) | 289 (m) | 293 (m+4) | 290 (m+1) | |
| Fructose | D-Fructose | [1,2,3-13C3] D-fructose | [U-13C6] D-fructose | ||
| C1-C6 | m/z = m | m/z = m+3 | m/z = m+6 | ||
| C1-C3 | 101 (m) | 104 (m+3) | 104 (m+3) | ||
| C1-C3 | 203 (m) | 206 (m+3) | 206 (m+3) | ||
| C4-C6 | 115 (m) | 115 (m) | 118 (m+3) | ||
| C4-C6 | 217 (m) | 217 (m) | 220 (m+3) | ||