Figure 2.
Serotonin and SERT immunoreactivity. A) Staining in dorsal horn in normal, MOD and SEV L5 spinal cord. Area fraction of immunoreactivity is indicated in bottom right corner of each micrograph. In normal rats, the area fraction of SERT staining is greater than that of 5-HT. Note the marked decrease in both 5-HT and SERT immunostaining in MOD and absence in SEV animals. B) Staining in ventral horn in normal, MOD and SEV L5 spinal cord. Area fraction of immunoreactivity is indicated in bottom right corner of each micrograph. In normal spinal cord, area fraction of SERT staining is greater than for 5-HT staining. Note decrease in immunoreactivitiy for both 5-HT and SERT in both contusion models.

