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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: FEBS Lett. 2009 Dec 17;584(3):619–624. doi: 10.1016/j.febslet.2009.12.011

Figure 2. Sharp-1 induces GADD45α and Brca1 expression.

Figure 2

(A) Cells were left untreated (−) or treated with doxycycline (+ Doxy) for 16 and 36 hr. Sharp-1, cyclin B1, phosphorylated cdc2 (Tyr 15), CDK2, p21waf/cip1 and p53 were analyzed by western blot. (B) GADD45α and Brca1 mRNA was analyzed by quantitative RT-PCR. GAPDH was used as an internal control. (C). The level of the mitotic marker H3 (ser 10) was determined before after doxycycline treatment by western blot. (D) Expression of RPA, 53BP1, and MDC1 was analyzed by RT-PCR. The results are representative of two independent experiments. (E) PCNA and GAPDH levels were analyzed by western blot.