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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Jul 17;1798(2):194–201. doi: 10.1016/j.bbamem.2009.07.007

Figure 1.

Figure 1

(a) HFP_K3-induced lipid mixing of LM3 LUVs at 37 °C with HFP_K3, total lipid, and cholesterol concentrations of 1.5, 150, and 75 μM, respectively. (b, c) REDOR-filtered magic angle spinning 13C NMR spectra of LM3-associated HFP and HFP_K3, respectively. The peptides were 13CO labeled at Phe-8 and 15N labeled at Leu-9 and the S0 – S1 spectra were dominated by the Phe-8 13CO signal whose peak chemical shift was consistent with β strand conformation. The samples contained 0.4 μmol peptide, 40 μmol total lipid, and 20 μmol cholesterol and were cooled with nitrogen gas at −50 °C. Each spectrum was the sum of ∼10000 acquisitions and was processed with 50 Hz line broadening.