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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Jul 17;1798(2):194–201. doi: 10.1016/j.bbamem.2009.07.007

Figure 2.

Figure 2

NMR spectra of lipid/cholesterol dispersions with peptides. 2H spectra are displayed in panels a-e and the samples contained LM3-DMPCdac dispersions with peptide and peptide:total lipid (excluding cholesterol) mol ratios: (a) no peptide; (b) HFP, 0.03; (c) HFP, 0.10; (d) melittin, 0.03; and (e) melittin, 0.10. 31P spectra are displayed in panels f-h and the samples contained LM3 dispersions with peptide and peptide:lipid mol ratios: (f) no peptide; (g) HFP, 0.02; and (h) HFP_K3, 0.02. A similar 31P spectrum is displayed in panel i and contained HFP:total lipid = 0.02 with the dispersion composed of DOPC:DOPE:cholesterol in a 1:1:1 mol ratio. A typical sample contained ∼30 μmol total lipid and ∼15 μmol cholesterol. The 2H and 31P spectra were obtained at 35 °C, were respectively processed with 150 or 50 Hz line broadening, and were respectively the sums of 4000 to 6000 scans or 5000 to 20000 scans.