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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Dec 6;391(1):1049–1055. doi: 10.1016/j.bbrc.2009.12.017

Fig. 2.

Fig. 2

(A) and (B) TWS119 induces differential activation of caspases 3 and 7 in Rh30 and RD cells. The activity of caspases 3 and 7 (A) was determined as described in Materials and methods. Data represent 3 independent experiments, each performed in triplicate. In the PARP cleavage assay (B), Rh30 and RD cells were exposed to TWS119 for 72 h before Western blot analysis, using anti-PARP antibody and anti β-actin antibody (as loading control). (C) TWS119 inhibits activation of GSK3. Rh30 and RD cells were exposed to DMSO or TWS119 for 24 h and analyzed by Western blot, using indicated specific antibodies. (D) and (E) PAX3-FKHR sensitizes TWS119-mediated inhibition of cell proliferation in RMS cells. Cells were exposed to indicated concentrations (D) or 5 μM (E) of TWS119 for 72 h and viable cells (%) was determined. Data represent 3 independent experiments, each performed in triplicate.