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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Dec 6;391(1):1049–1055. doi: 10.1016/j.bbrc.2009.12.017

Fig. 4.

Fig. 4

GSK3 phosphorylates PAX3-FKHR in vitro. HEK293T cells were transiently transfected with EGFP-vector or EGFP-PAX3-FKHR plasmids. (A) The amount of EGFP and EGFP-PAX3-FKHR (EGFP-PF) proteins immunoprecipitated by anti-GFP agarose beads were revealed by staining with SimplyBlue. Purified GFP (Millipore) was included as a control. (B) In vitro kinase assays. (C) EGFP and EGFP-PF were revealed by Western blot, using anti-GFP antibody.