Fig. 3. Effects of tubercidin and SAM:SAH ratios on proteasome activity in cytosol and nuclear fractions.

A. Huh7CYP cells were exposed for 24h to 10 μM tubercidin and then proteasome activity was measured in the prepared cytosolic and nuclear fractions. * is p<0.05 in proteasome activity in nuclear extracts, controls vs tubercidin; # is p<0.05 in proteasome activity in cytosols, control vs tubercidin. B. proteasome activity in liver cytosols exposed to low SAM:SAH ratio (2) for 4 hr. * is p<0.05 in proteasome activity between control and treatments.