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. Author manuscript; available in PMC: 2011 Jan 8.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Dec 21;391(2):1291–1296. doi: 10.1016/j.bbrc.2009.12.074

Fig. 4. Effects of various SAM:SAH ratios on proteasome activity in purified preparations of A.

Fig. 4

20S proteasome (representative experiment) and B. 20S proteasome vs IPr (immunoproteasome). 20S proteasome or IPr was incubated for 2h in buffers with varying SAM:SAH ratios and then proteasome activity was measured as detailed. * is p<0.05 difference in the activity between 20S proteasome and IPr and # is p<0.05 between control and other treatments (shown as % control, where the activity of both 20S proteasome and IPr in control was considered as 100%). C. Expression of methyl lysine in 20S proteasome under various SAM:SAH ratios. Purified 20S proteasome was incubated for 15 min in buffers with SAM:SAH ratio 5 or 2.5 and then immunoblotted and probed with antibody to methyl lysine. A 25kDa proteasome subunit showed positive reactivity with anti-methyl lysine. 1-no treatment; 2- treated with SAM:SAH ratio 5; 3-treated with SAM:SAH ratio 2.5.