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. Author manuscript; available in PMC: 2011 Jan 26.
Published in final edited form as: Carbohydr Res. 2009 Nov 3;345(2):250–256. doi: 10.1016/j.carres.2009.10.024

Figure 2.

Figure 2

500-MHz 1H NMR spectra of [15N]N-acetylheparosan. (A) One-dimensional 1H NMR spectrum of [15N]N-acetylheparosan with waltz decoupling of 15N during acquisition. The spectrum reveals a single peak centered at 8.21 ppm that is otherwise split by 15N when recorded in the absence of 15N decoupling (B). The measured one-bond scalar coupling (1JNH) is 91.7 Hz, consistent with the expected value of amides. A single asterisk (*) indicates the signal arising from a small population for amide protons attached to 14N, indicating incomplete 15N incorporation, and is furthermore consistent with the 94% isotopic purity of [15N]N-acetylheparosan calculated by ESIMS. The double asterisks (**) indicates the residual water signal. (C) Two-dimensional (15N, 1H) HSQC shows a single correlation at 1H, 8.21 and 15N, 123.3 ppm.