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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Nov 10;391(1):209–213. doi: 10.1016/j.bbrc.2009.11.033

Fig. 1.

Fig. 1

Effect of mqsR and mqsA mutations on persister cell formation. BW25113 wild-type, BW25113 mqsR, and BW25113 mqsR mqsA were grown to a turbidity of 0.5 at 600 nm in LB medium at 37°C, adjusted on a turbidity of 1, and exposed to 100 μg/mL ampicillin for 2 h (A). Persister cell formation of BW25113/pCA24N, BW25113/pCA24N-mqsR, BW25113/pBS(Kan), BW25113/pBS(Kan)-mqsA BW25113/pBS(Kan)-mqsR, and BW25113/pBS(Kan)-mqsR-mqsA grown to a turbidity of 0.5 in LB medium at 37°C, contacted with 1 mM IPTG for 2 hr for induction, adjusted to a turbidity of 1, and exposed to 100 μg/mL ampicillin for 2 h in LB (B). Persister data are the average of two independent cultures, and one standard deviation is shown. The asterisk indicates statistical significance determined using a Student's t-test (p < 0.05).