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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Anal Biochem. 2009 Oct 9;397(2):139–143. doi: 10.1016/j.ab.2009.10.009

Figure 3.

Figure 3

Thiophosphate transfer from histidine kinases to response regulators. A) reaction with ATP32P showing phosphate transfer from the histidine kinase EnvZ to MBP-tagged OmpR. Lane 1: EnvZ, 2: MBP-OmpR alone, 3: MBP-OmpR + EnvZ [5 µM EnvZ, 5 µM MBP-OmpR]. B) reaction using ATPγS and the antibody assay showing thiophosphate transfer from the histidine kinase EnvZ to MBP-tagged OmpR. Lane 1: EnvZ, 2: MBP-OmpR alone, 3: MBP-OmpR + EnvZ [5 µM EnvZ, 5 µM MBP-OmpR]. C) thiophosphate transfer from the H-NOX associated histidine kinase in Hahella chejuensis to the response regulator found in the same predicted operon. Lane 1: Hahella histidine kinase, 2: Hahella response regulator alone, 3: Hahella histidine kinase + response regulator [20 µM Hahella histidine kinase, 5 µM Hahella response regulator].