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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Arch Biochem Biophys. 2009 Oct 23;493(2):125–134. doi: 10.1016/j.abb.2009.10.011

Fig. 5.

Fig. 5

Organellar localization of IDP1+CKL. [A] Yeast idp1Δidp2Δidp3Δ transformants expressing plasmid-borne copy of the IDP1+CKL gene were grown using YP oleate or petroselinate medium as indicated to A600nm = 2. Cellular, cytosolic, and organellar protein extracts (5 μg each) prepared from transformants were electrophoresed for immunoblot analysis using an antiserum for IDP1/IDP2 [7] or an antiserum for peroxisomal MDH3 [24]. [B] Quantitative results are shown for densitometric analyses of multiple immunoblots (similar to those shown in [A]) used to visualize organellar levels of IDP3 in the parental strain and of IDP1+CKL in transformed mutant strains (idp1Δ, Δidp3, and idp1Δidp3Δ) used for complementation analyses. Organellar levels were quantified relative to samples of purified IDP1 and IDP3 as described under Experimental Procedures.