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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Exp Neurol. 2009 Oct 31;221(1):166–174. doi: 10.1016/j.expneurol.2009.10.019

Figure 1.

Figure 1

Effects of PPBP on neuronal damage and neurologic deficits in piglets subjected to hypoxia-ischemia (H–I). Piglets exposed to H–I or sham surgery were infused intravenously with saline, low-dose PPBP (PPBP-Low), or high-dose PPBP (PPBP-High). (A) Representative photographs of H&E-stained sections show that low and high doses of PPBP alleviate ischemic neuronal damage in putamen at 4 days of recovery. Arrows point to representative ischemia-damaged neurons and arrow head shows the normal neurons. Scale bar=40 µm and scale bar in insert = 8 µm. (B) Western blot and double-immunofluorescent results show that sigma-1 receptors exist in pig striatum and are localized mainly in neurons. Ponceau S was used as a loading control. Scale bar = 8 µm. (C) Quantitative results for viable putamen neurons expressed as a percent of the mean value of the sham+saline group. (D) Neurologic scores during the 4-day recovery. Data represent means ± s.d. (n = 4 to 10 per group). *P < 0.05 versus sham+saline group; #P < 0.05 versus H–I+saline group; ANOVA followed by the Student-Newman-Keuls test.