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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Exp Neurol. 2009 Oct 31;221(1):166–174. doi: 10.1016/j.expneurol.2009.10.019

Figure 4.

Figure 4

The impact on association of NR2 with nNOS by H–I and PPBP treatment involves changes in coupling of nNOS to PSD-95, but not PSD-95 to NR2. Immunoprecipitation of membrane-enriched fraction of putamen (A) or prefrontal cortex (B) at 3 h of recovery with antibody against PSD-95 results in the co- precipitation of nNOS and NR2. H–I increased the interaction between nNOS and PSD-95 [as seen by an increase in optical density (OD) ratio] only in putamen. This increase was restored to control level by PPBP treatment. (C) Western blot analysis showed that H–I induced nNOS Ser847 phosphorylation in the membrane-enriched fraction at 3 h of recovery; the induction was prevented by PPBP treatment. The H–I– induced increase in phosphorylated Ser847 was proportional to the increase in total membrane nNOS, resulting in no change in the relative amount of phosphorylated protein. All data are shown as means ± s.d., normalized to the sham+saline value. *P < 0.05 versus sham+saline group; #P < 0.05 versus H–I+saline group; one-way ANOVA followed by the Student-Newman-Keuls test.