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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Exp Neurol. 2009 Oct 31;221(1):166–174. doi: 10.1016/j.expneurol.2009.10.019

Figure 5.

Figure 5

PPBP treatment reduces H–I–induced oxidative and nitrative stress in putamen at 3 h of recovery. Representative sections of immunohistochemical staining for 8-hydroxy-2-deoxyguanosine (OHdG)/8-hydroxy-guanosine (OHG) (A) and 8-nitroguanosine (Nitro-G) (B) in putamen of piglets at 3 h of recovery after sham surgery or H–I. Faint staining of cell bodies was present in sham-operated piglets, whereas elevated signals could be observed in both cytosol and nucleus. Inset: representative Nitro-G signal in nucleus. Scale bar = 50 µm; inset scale bar = 10 µm. (C) H–I increased optical density (OD) of OHdG/OHG and Nitro-G positive cells in putamen at 3 h of recovery; both were attenuated by PPBP treatment (n = 85 to 150 cells per group). (D) Western blot analysis showed that PPBP decreased the H–I–induced 3-nitrotyrosine immunoreactivity on multiple protein bands in putamen of sham-operated and H–I piglets at 3 h of recovery. All data are shown as mean ± s.d. *P < 0.05 versus sham+saline group; #P < 0.05 versus H–I+saline group; one-way ANOVA followed by the Student-Newman-Keuls test.