Table 1.
Training parameters.
| Phase | # of Sessions | LH (s) | TO (s) | Stimuli | Stimulus Presentation |
|---|---|---|---|---|---|
| Training | |||||
| Spout | 3 | N/A | N/A | H2O | Constant |
| Side | 6 | 180 | 0 | 0.2M NaCl or KCl | Constant |
| Alternation | 4 | 15 | 10 | 0.2M NaCl and KCl | Criteriona |
| Discrimination I | 3–4 | 10 | 10 | 0.2M NaCl and KCl | Semi-randomb |
| Discrimination II | 3–5 | 10 | 20 | 0.1, 0.2, and 0.4 M NaCl and KCl | Semi-random |
| Discrimination III | 3–4 | 5 | 30 | 0.1, 0.2, and 0.4 M NaCl and KCl | Semi-random |
| Presurgical Testing | 10 | 5 | 30 | 0.1, 0.2, and 0.4 M NaCl and KCl [with or without 100μM amiloride] | Semi-random |
| Postsurgical Testing | 30 | 5 | 30 | 0.1, 0.2, and 0.4 M NaCl and KCl [with or without 100μM amiloride] | Semi-random |
Note: LH = limited hold, the amount of time (in seconds) the rat has to respond after sampling the stimulus. TO = time out (in seconds) for incorrect responses.
the alternation criteria used: 8, 6, 4, 2.
Stimuli were presented in randomized blocks of 6 without replacement.