Table 1.
Composition of Pre-Permeabibzation Medium and Respiration Medium developed by Safiulina et al. [37]. Concentrations given in mM unless otherwise noted.
Pre-Permeabilization Medium | Respiration Medium | |
---|---|---|
Potassium Methanesulfonate | 81 | |
BES | 60 | |
Mg2ATP | 5.69 | |
KOH | 35 | |
K-MES | 100 | |
Imidazole | 20 | |
MgCl2 | 1.38 | |
HC1 | 16.25 | |
BSA | 5 mg/mL | |
K2EGTA* | 7.23 | 7.23 |
CaK2EGTA* | 2.77 | 2.77 |
Taurine | 20 | 20 |
K2HPO4 | 3 | 3 |
Dithiothreitol | 0.5 | 0.5 |
pH | 7.1 | 7.1 |
Osmolarity | 255 mOsm | 225 mOsm |
Free [Ca2+] in both media was 0.1 μM. K2EGTA and Ca2EGTA were made according to Oroboros (www.oroboros.at, Innsbruck, Austria).