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. Author manuscript; available in PMC: 2011 Jan 15.
Published in final edited form as: Dev Biol. 2009 Nov 18;337(2):425–431. doi: 10.1016/j.ydbio.2009.11.019

Figure 2. Location and time of differentiation of larval melanocytes.

Figure 2

(A) Cartoon showing regions of embryonic and larval melanocyte quantitation. (B–E) Photos of Tg(Tyrp1:GFP)j900 larva at 5 dpf after treatment with PTU from 3–5 dpf showing melanocytes that differentiated prior to melanin inhibition by PTU (melanin+, GFP+, red arrowheads) and melanocytes that differentiated after melanin inhibition by PTU (melanin-, GFP+, red arrows). (B, C) Dorsal view of the head. (D, E) Lateral view showing the left-side lateral stripe. Additional, out-of-focus fluorescence is also apparent from melanocytes in the right-side horizontal myoseptum. (F) Quantitation of embryonic melanocytes in various regions at 3 dpf. Average number of melanized cells, error bars represent standard deviation, N=10. (G) Quantitation of melanocytes that develop from 3–5 dpf, 5–7 dpf, and 7–10 dpf in these regions. Average number of GFP-positive, melanin-negative cells at time of assay, error bars represent standard deviation, N=10. Scale bars 100 um.