Fig. 3.

Intracellular calcium mobilization is significantly attenuated after PF-228 treatment. Platelets were loaded with Fura2-AM and were either pre-treated with DMSO (−) or PF-228 (+) (1 μM) for 5 min before addition of either thrombin (0.1 U/ml; A) or CRP (5 μg/ml; B). Representative calcium traces are shown. The mean peak height (±SEM; n = 3) was measured and expressed as a percentage of the control, i.e. agonist alone.