Fig. 4.

ADP partially rescues the aggregation defect after treatment with PF-228. Platelets were either pre-treated with DMSO (−) or PF-228 (+) (1 μM) for 5 min before the addition of CRP (5 μg/ml), or a combination of CRP (5 μg/ml) and ADP (10 μM), and aggregation monitored (A). Representative aggregation traces are shown. Aggregation after different treatments was determined and normalised to the maximal aggregation induced by CRP (5 μg/ml) and ADP (10 μM). The data was presented as mean percentage of maximal aggregation (±SEM; n = 3).