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. 2010 Jan 11;188(1):145–156. doi: 10.1083/jcb.200908082

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© 2010 Fernández-Busnadiego et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Segmentation procedure. (A) 2.7-nm-thick tomographic slice of a 100-mM HTS-treated synaptosome. The dashed line indicates the analyzed area (first 250 nm from the AZ). (B) 3D segmentation of synaptic vesicles (yellow), AZ (gray), synaptic vesicle connectors (red), and synaptic vesicle tethers (blue). Boxed regions are magnified in C and D. (C and D, top) Tomographic slices showing a synaptic vesicle connector (C) and a tether (D) and their 3D visualization as detected by the automated segmentation procedure (bottom). Bars: (A) 200 nm; (C and D) 50 nm.