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. 2010 Jan 11;188(1):101–114. doi: 10.1083/jcb.200904075

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© 2010 Yen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — The COG complex is required for autophagosome formation. (A) The wild-type (WT; pep4Δ vps4Δ; FRY143), atg1Δ (JHY28), and cog2-1 (WLY221) strains were grown in YPD at 24°C to OD₆₀₀ = 0.8, shifted to SD-N for 1.5 h at 37°C, and prepared for electron microscopy analysis as described in Materials and methods. Bars, 0.5 µm. (B) Quantification of autophagic body accumulation. The number of autophagic bodies in 54 or 75 cells containing vacuoles of similar size in wild-type and cog2-1 cells, respectively, was quantified. (C) Quantification of the diameter of autophagic bodies (AB). The diameter of autophagic bodies in wild-type (n = 45) and cog2-1 (n = 37) cells was measured and quantified. Error bars represent SD.