Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Jan 11;188(1):101–114. doi: 10.1083/jcb.200904075

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Yen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 7. — COG subunits associate with Atg proteins. (A and B) HA-Cog4 (A; WLY208) and HA-Cog2 (B; WLY209) cells transformed with the indicated plasmids expressing tagged Atg proteins were grown in selective SMG medium to OD₆₀₀ = 1.0. Cell lysates were prepared and subjected to affinity isolation or immunoprecipitation (IP) with either anti-HA or anti-Myc antibody as described in Materials and methods. Plasmids expressing PA (pRS424-CuProtA), PA-tagged Atg17 (pProtA-Apg17(424)), Atg20 (pProtA-Cvt20(424)), Atg24 (pProtA-Cvt13(424)), and Myc-tagged Atg12 (pMyc-Apg12(426)) were used as indicated. The eluted proteins were separated by SDS-PAGE and detected with monoclonal anti-HA antibody (A) or immunoblotting with anti-HA and anti-Myc antibodies (B). For each experiment, ∼1% of the total cell lysate or 10% of the total eluate was loaded. WB, Western blot. (C) Cog2-GFP colocalizes with RFP-Ape1 in the MKO (ATG11 ATG19) strain. The MKO (ATG11 ATG19; WLY205) cells expressing chromosomally tagged Cog2-GFP and a plasmid-based RFP-Ape1 were grown in selective SMD to OD₆₀₀ = 0.8 and observed by fluorescence microscopy. DIC, differential interference contrast. Bar, 2.5 µm.