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. 2010 Jan 11;188(1):83–100. doi: 10.1083/jcb.200906147

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© 2010 van Ree et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Transgenic UbcH10 protein is overexpressed throughout the cell cycle. (A) Immunostaining of wild-type and UbcH10^T2/T2 MEFs with anti-UbcH10 antibody. DNA was visualized with Hoechst. (B) Western blot analysis of extracts from wild-type and UbcH10^T2/T2 MEFs arrested in mitosis with nocodazole and released in fresh medium for the indicated times. Blots were probed for UbcH10 to monitor its degradation during mitotic exit. Cyclin B and P-H3 were markers for mitotic exit. (C) Analysis of UbcH10 levels in wild-type and UbcH10^T2/T2 MEFs at various times after release from serum starvation. Cyclin A2 was a marker for S-phase entry. Bar, 10 µm.