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Biophysical and biochemical properties of ribonucleases
| Ribonuclease | Tm (°C)a | kcat/KM (M−1s−1)b |
|---|---|---|
| RNase A | 63 | (4.3 ± 0.3) × 107 |
| fluorescein–RNase A | 61 | (0.54 ± 0.02) × 107 |
| ONC | 90 | (3.5 ± 0.1) × 102 |
| DTNB–ONC | 88 | (1.7 ± 0.2) × 102 |
Values of Tm (± 1 °C) were determined in PBS by UV spectroscopy.
Values of kcat/KM (± SE) are for the catalysis of 6-FAM–dArU(dA)2–6-TAMRA cleavage at (23 ± 2) °C in 0.10 M MES–NaOH buffer (pH 6.0) containing NaCl (0.10 M).
